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LB肉湯干粉的配方成分

發(fā)布時(shí)間:

2022-12-27

作者:

日水生物


LB肉湯干粉培養(yǎng)基的配制方法:稱取本品20.0g于1L蒸餾水或去離子水中(可按比例增加或減少配制量),加熱溶解,分裝,121℃高壓滅菌15分鐘,備用。
 
Formula(配方): g/L
 
Peptone/蛋白胨:10.0g
Yeast Extract/酵母浸粉:5.0g
Sodium Chloride/氯化鈉:5.0g        
pH7.0±0.2(25℃) 
The preparation method of LB broth dry powder medium: This product is called 20.0g in 1L distilled water or deionized water (can increase or reduce the amount of preparation according to proportion), heat dissolve, separate, sterilize at 121 centigrade for 15 minutes, spare.
Formula: g/L
Peptone: 10.0g
Yeast extract: 5.0g
Sodium chloride: 5.0g
PH7.0 + 0.2 (25 C)