改良Letheen瓊脂基礎(chǔ)配方
發(fā)布時間：
2022-12-27
作者：
改良Letheen瓊脂基礎(chǔ)配制方法：稱取本品52.1g于1L蒸餾水或去離子水中,加熱溶解后加入7g吐溫-80,搖勻,分裝,121℃高壓滅菌15分鐘，滅菌后請搖勻,以防瓊脂沉積于器皿底部而凝固，備用。
Letheen Agar Base Modified Formula[配方]： g/L
Tryptone [胰蛋白胨]：10.0g
Pancreatic Digest of Casein[胰酪蛋白胨]：5.0g
Proteose[月示蛋白胨]：10.0g
Sodium Chloride[氯化鈉] ：5.0g
Beef Extract[牛肉浸粉]：3.0g
Yeast Extract[酵母浸粉]：2.0g
Dextrose[葡萄糖]：1.0g
Sodium Bisulfite[亞硫酸氫鈉]：0.1g
Lecithin[卵磷脂] ：1.0g
Agar[瓊脂]：15.0g
pH7.2±0.2[25℃]
Modified Letheen agar basic preparation method: weigh 52.1g in 1L distilled water or deionized water, add 7g Tween-80 after heating and dissolving, shake well, pack, high-pressure sterilization at 121 C for 15 minutes, shake well after sterilization, in order to prevent agar deposition in the bottom of the vessel and solidify, reserve.
Letheen Agar Base Modified Formula[formula]:g/L
Tryptone [tryptone]:10.0g
Pancreatic Digest of Casein[casein peptone]:5.0g
Proteose[peptone]:10.0g
Sodium Chloride[NaCl]:5.0g
Beef Extract[beef dip powder]:3.0g
Yeast Extract[yeast extract powder]:2.0g
Dextrose[glucose]:1.0g
Sodium Bisulfite[Sodium Bisulfite]:0.1g
Lecithin[lecithin]:1.0g
Agar[agar]:15.0g
PH7.2 + 0.2[25 C]