TGC流體培養(yǎng)基配方說(shuō)明
發(fā)布時(shí)間：
2022-12-27
作者：
青島日水生物培養(yǎng)基
TGC流體培養(yǎng)基配制方法：稱取本品29.3g于1L于蒸餾水或去離子水中[可按比例增加或減少配制量]，加熱煮沸至完全溶解，分裝，121℃高壓滅菌15分鐘，滅菌結(jié)束后迅速冷卻，備用。
Formula[配方]： g/L
L-Cystine[L-胱氨酸]：0.5g
Peptone[蛋白胨] ：15.0g
Yeast Extract[酵母浸粉]：5.0g
Sodium Chloride[氯化鈉]：2.5g
Dextrose[葡萄糖] ：5.0g
Sodium Thioglycollate[硫乙醇酸鈉]：0.5g
Resazurin[刃天青] ：0.001g
Agar[瓊脂] ：0.75g
pH7.1±0.2[25℃]
TGC fluid medium preparation method: weigh this product 29.3g in 1L distilled water or deionized water [can increase or reduce the amount of preparation in proportion], heat and boil until completely dissolved, sub-packed, high-pressure sterilization at 121 C for 15 minutes, after sterilization quickly cooling, reserve.
Formula[formula]:g/L
L-Cystine[L- cystine]:0.5g
Peptone[peptone]:15.0g
Yeast Extract[yeast extract powder]:5.0g
Sodium Chloride[NaCl]:2.5g
Dextrose[glucose]:5.0g
Sodium Thioglycollate[]:0.5g sodium glycolate
Resazurin[azure]:0.001g
Agar[agar]:0.75g
PH7.1 + 0.2[25 C]